Hotspot

rpoB

LSC · 26625-29837 · 3213 bp

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Pi 0.00336

MarkerSeek score 38.40

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3213
variable_sites: 27
pi: 0.00336
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	TGGCATTTTCTTTTGACCCG	AAAGCAAGGATATGGGCTCG	45.0	50.0	57.2	58.0	175-176 bp	1.0	76.0
rpoB_p2	GGCATTTTCTTTTGACCCGA	AAAGCAAGGATATGGGCTCG	45.0	50.0	56.9	58.0	174-175 bp	1.0	73.7
rpoB_p3	ATGGCATTTTCTTTTGACCCG	AAAGCAAGGATATGGGCTCG	42.9	50.0	57.7	58.0	176-177 bp	1.0	72.0
rpoB_p4	CGCCCACACAAGCAAATTG	AAAGCAAGGATATGGGCTCG	52.6	50.0	58.5	58.0	211-212 bp	1.0	70.2
rpoB_p5	GGCATTTTCTTTTGACCCGAT	AAAGCAAGGATATGGGCTCG	42.9	50.0	57.4	58.0	174-175 bp	1.0	69.8