Hotspot

ndhA

SSC · 127802-130333 · 2532 bp

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Pi 0.00421

MarkerSeek score 52.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2532
variable_sites: 21
pi: 0.00421
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAAGGAACA	GTCCCCCGGGTTTTATCAAT	42.9	50.0	56.3	57.6	2753-2770 bp	1.0	55.3
ndhA_p2	AACCCAGTTACCATAAGGAACA	GTCCCCCGGGTTTTATCAAT	40.9	50.0	57.0	57.6	2754-2771 bp	1.0	53.3
ndhA_p3	TGAACCCAGTTACCATAAGGA	GTCCCCCGGGTTTTATCAAT	42.9	50.0	56.0	57.6	2756-2773 bp	1.0	53.1
ndhA_p4	GAACCCAGTTACCATAAGGAACA	GTCCCCCGGGTTTTATCAAT	43.5	50.0	58.1	57.6	2755-2772 bp	1.0	52.6
ndhA_p5	TGAACCCAGTTACCATAAGGAAC	GTCCCCCGGGTTTTATCAAT	43.5	50.0	58.1	57.6	2756-2773 bp	1.0	52.6