Hotspot

ndhF-rpl32

SSC · 120595-121537 · 943 bp

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Pi 0.00297

MarkerSeek score 61.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 943
variable_sites: 6
pi: 0.00297
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CGGGAAAAAGGAGAAGTCCC	TGCTACCCAATATGCCTTCT	55.0	45.0	58.2	56.3	1117-1119 bp	1.0	65.4
ndhF-rpl32_p2	CGGGAAAAAGGAGAAGTCCC	TCAATGCTACCCAATATGCCT	55.0	42.9	58.2	57.1	1121-1123 bp	1.0	64.1
ndhF-rpl32_p3	CGGGAAAAAGGAGAAGTCCC	TGCTACCCAATATGCCTTCTT	55.0	42.9	58.2	57.0	1117-1119 bp	1.0	63.5
ndhF-rpl32_p4	CGGGAAAAAGGAGAAGTCCC	GCTACCCAATATGCCTTCTTCT	55.0	45.5	58.2	57.9	1116-1118 bp	1.0	62.4
ndhF-rpl32_p5	CGGGAAAAAGGAGAAGTCCC	ACCCAATATGCCTTCTTCTTCC	55.0	45.5	58.2	58.1	1113-1115 bp	1.0	62.4