Hotspot

accD-psaI

LSC · 65499-66113 · 615 bp

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Pi 0.00146

MarkerSeek score 56.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 615
variable_sites: 2
pi: 0.00146
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGATTCAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	773-798 bp	1.0	82.2
accD-psaI_p2	TTGATTCAATCGTACCGCGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	779-804 bp	1.0	81.6
accD-psaI_p3	TCAATCGTACCGCGTAATCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	768-793 bp	1.0	81.2
accD-psaI_p4	CAATCGTACCGCGTAATCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	767-792 bp	1.0	81.2
accD-psaI_p5	TCAATCGTACCGCGTAATCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.9	57.9	774-799 bp	1.0	80.6