Hotspot

atpB-rbcL

LSC · 60727-61516 · 790 bp

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Pi 0.0137

MarkerSeek score 61.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 790
variable_sites: 27
pi: 0.0137
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CCTGGGGGAAAAGCTACATC	AACACCAGCTTTGAACCCAA	55.0	45.0	57.9	58.1	952-955 bp	1.0	80.8
atpB-rbcL_p2	CTGGGGGAAAAGCTACATCC	AACACCAGCTTTGAACCCAA	55.0	45.0	57.9	58.1	951-954 bp	1.0	80.8
atpB-rbcL_p3	GGGGGAAAAGCTACATCCAG	AACACCAGCTTTGAACCCAA	55.0	45.0	57.9	58.1	949-952 bp	1.0	80.8
atpB-rbcL_p4	AAGCTACATCCAGTACCGGA	AACACCAGCTTTGAACCCAA	50.0	45.0	57.8	58.1	942-945 bp	1.0	80.1
atpB-rbcL_p5	ATTTGAGCGATACGTCCCAG	AACACCAGCTTTGAACCCAA	50.0	45.0	57.8	58.1	916-919 bp	1.0	79.6