Hotspot

psaA-ycf3

LSC · 47189-48030 · 842 bp

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Pi 0

MarkerSeek score 53.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 842
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	943-988 bp	1.0	82.9
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	949-994 bp	1.0	82.6
psaA-ycf3_p3	AGAAATGACCGGGTTTAGCC	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	961-1006 bp	1.0	82.6
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	974-1019 bp	1.0	82.5
psaA-ycf3_p5	AGAAATGACCGGGTTTAGCC	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	967-1012 bp	1.0	82.3