Hotspot

atpF-atpH

LSC · 15172-15773 · 602 bp

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Pi 0.00266

MarkerSeek score 53.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 602
variable_sites: 4
pi: 0.00266
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	TGGGCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	719-739 bp	1.0	76.3
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	AGGCAGAGGGAAAAATACGG	50.0	50.0	57.9	57.6	786-806 bp	1.0	76.2
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATACGGGG	50.0	55.0	57.9	58.6	784-804 bp	1.0	74.7
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GGCAGAGGGAAAAATACGGG	50.0	55.0	57.9	58.6	785-805 bp	1.0	74.7
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CCGAGGCAGAGGGAAAAATA	50.0	50.0	57.9	57.3	789-809 bp	1.0	73.9