Hotspot

trnR-UCU-atpA

LSC · 11803-12293 · 491 bp

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Pi 0.00384

MarkerSeek score 55.60

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 491
variable_sites: 4
pi: 0.00384
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	AAGACATTCACTCGGGAAGC	47.6	50.0	57.3	57.9	587-611 bp	1.0	69.1
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	CCAAGACATTCACTCGGGAA	47.6	50.0	57.3	57.5	589-613 bp	1.0	66.2
trnR-UCU-atpA_p3	TGGATAGGACAGAGGTCTTCT	AAGACATTCACTCGGGAAGC	47.6	50.0	56.9	57.9	622-646 bp	1.0	66.1
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ACCAAGACATTCACTCGGGA	47.6	50.0	57.3	58.7	590-614 bp	1.0	64.7
trnR-UCU-atpA_p5	GTTCAAATCCTATTGGACGCA	AAGACATTCACTCGGGAAGC	42.9	50.0	56.6	57.9	586-610 bp	1.0	63.5