Hotspot

trnS-GCU-trnG-GCC

LSC · 9420-10611 · 1192 bp

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Pi 0.0052

MarkerSeek score 65.50

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1192
variable_sites: 13
pi: 0.0052
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.0	57.3	1244-1311 bp	1.0	79.1
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	TGAATCAAACGAGGGATCCC	50.0	50.0	58.0	57.3	1243-1310 bp	1.0	79.1
trnS-GCU-trnG-GCC_p3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.2	57.3	1298-1365 bp	1.0	77.7
trnS-GCU-trnG-GCC_p4	TGGAAACGGAAAGAGAGGGA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.3	57.3	1303-1370 bp	1.0	76.7
trnS-GCU-trnG-GCC_p5	CAATCCGACGCTTTAGTCCA	GGATCCATAGAACGAATCGCA	50.0	47.6	58.0	58.0	1212-1279 bp	1.0	76.6