Hotspot

rps16-trnQ-UUG

LSC · 7021-7791 · 771 bp

Back to Neltuma

Pi 0.00363

MarkerSeek score 53.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 7
pi: 0.00363
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	845-851 bp	1.0	80.6
rps16-trnQ-UUG_p2	ATGTCTTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	847-853 bp	1.0	79.5
rps16-trnQ-UUG_p3	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	833-839 bp	1.0	79.1
rps16-trnQ-UUG_p4	CATGTCTTTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	848-854 bp	1.0	78.9
rps16-trnQ-UUG_p5	TGTCTTTCAAGTCGCACGTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.4	58.1	846-852 bp	1.0	77.8