Hotspot

ycf1

SSC · 115345-121026 · 5682 bp

Back to Nekemias

Pi 0.00273

MarkerSeek score 54.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5682
variable_sites: 30
pi: 0.00273
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGGTCGATATTGAGGTCTGGA	GAACCAAGGTTCCAGATTCGA	47.6	47.6	57.7	57.9	218 bp	1.0	64.4
ycf1_p2	AGGTCGATATTGAGGTCTGGA	AACCAAGGTTCCAGATTCGA	47.6	45.0	57.7	56.8	217 bp	1.0	62.9
ycf1_p3	AGGTCGATATTGAGGTCTGGA	ACCAAGGTTCCAGATTCGAT	47.6	45.0	57.7	56.5	216 bp	1.0	61.0
ycf1_p4	AGGTCGATATTGAGGTCTGGA	GAACCAAGGTTCCAGATTCG	47.6	50.0	57.7	56.4	218 bp	1.0	60.2
ycf1_p5	GGTCGATATTGAGGTCTGGA	GAACCAAGGTTCCAGATTCGA	50.0	47.6	56.1	57.9	217 bp	1.0	60.0