Hotspot

clpP

LSC · 75399-77475 · 2077 bp

Back to Nekemias

Pi 0.00273

MarkerSeek score 59.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2077
variable_sites: 11
pi: 0.00273
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCACGGATTTGCACGAAATC	ACGTTTCCATACCAAAGTGA	45.0	40.0	57.1	54.9	2156-2162 bp	1.0	48.8
clpP_p2	TCACGGATTTGCACGAAATC	ACGTTTCCATACCAAAGTGAA	45.0	38.1	57.1	55.7	2156-2162 bp	1.0	47.3
clpP_p3	TCACGGATTTGCACGAAATC	ACGTTTCCATACCAAAGTGAAA	45.0	36.4	57.1	56.5	2156-2162 bp	1.0	45.3
clpP_p4	AAATCACGGATTTGCACGAA	ACGTTTCCATACCAAAGTGA	40.0	40.0	56.6	54.9	2159-2165 bp	1.0	45.0
clpP_p5	AATCACGGATTTGCACGAAA	ACGTTTCCATACCAAAGTGA	40.0	40.0	56.6	54.9	2158-2164 bp	1.0	45.0