Hotspot

ccsA

SSC · 129495-130463 · 969 bp

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Pi 0.00413

MarkerSeek score 53.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 7
pi: 0.00413
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	ATGGTTCTGGTTTTTCCCGG	CCGATTTCTATTCCGGGATCT	50.0	47.6	58.4	57.0	1116-1117 bp	1.0	61.9
ccsA_p2	ATGGTTCTGGTTTTTCCCGG	CCGATTTCTATTCCGGGATCTT	50.0	45.5	58.4	57.7	1116-1117 bp	1.0	59.4
ccsA_p3	ATGGTTCTGGTTTTTCCCGG	CCGATTTCTATTCCGGGATC	50.0	50.0	58.4	55.5	1116-1117 bp	1.0	57.5
ccsA_p4	GTTCTGGTTTTTCCCGGCTT	CCGATTTCTATTCCGGGATCT	50.0	47.6	59.0	57.0	1113-1114 bp	1.0	57.2
ccsA_p5	TTCTGGTTTTTCCCGGCTTG	CCGATTTCTATTCCGGGATCT	50.0	47.6	59.0	57.0	1112-1113 bp	1.0	57.2