Hotspot

rps19-rpl2

IRb · 89861-90113 · 253 bp

Back to Nekemias

Pi 0.0178

MarkerSeek score 65.90

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 253
variable_sites: 8
pi: 0.0178
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGGTAGATGTCCTGGACCAA	CACAACCCCTTGGGGTTATC	50.0	55.0	57.7	58.2	441-701 bp	1.0	80.8
rps19-rpl2_p2	TGGTAGATGTCCTGGACCAA	TTGGGGTTATCCTGCACTTG	50.0	50.0	57.7	57.8	432-692 bp	1.0	80.4
rps19-rpl2_p3	ATGGTAGATGTCCTGGACCA	CACAACCCCTTGGGGTTATC	50.0	55.0	57.4	58.2	442-702 bp	1.0	79.0
rps19-rpl2_p4	ATGGTAGATGTCCTGGACCA	TTGGGGTTATCCTGCACTTG	50.0	50.0	57.4	57.8	433-693 bp	1.0	78.6
rps19-rpl2_p5	TGGTAGATGTCCTGGACCAA	GGGTTATCCTGCACTTGGAA	50.0	50.0	57.7	57.5	429-689 bp	1.0	78.1