Hotspot

rpl22-rps19

IRb · 89250-89581 · 332 bp

Back to Nekemias

Pi 0.0151

MarkerSeek score 49.20

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 332
variable_sites: 10
pi: 0.0151
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TGATCAAGTACTCTTCGCGC	TACTCGAAATTTCCGGGGAC	50.0	50.0	58.1	57.3	419-440 bp	1.0	75.7
rpl22-rps19_p2	AAGTACTCTTCGCGCTTTGT	TACTCGAAATTTCCGGGGAC	45.0	50.0	57.8	57.3	414-435 bp	1.0	75.3
rpl22-rps19_p3	CGCTCGATAAGGCATGAGTT	TACTCGAAATTTCCGGGGAC	50.0	50.0	58.1	57.3	480-501 bp	1.0	75.1
rpl22-rps19_p4	GATCAAGTACTCTTCGCGCT	TACTCGAAATTTCCGGGGAC	50.0	50.0	57.8	57.3	418-439 bp	1.0	74.9
rpl22-rps19_p5	TACTCTTCGCGCTTTGTGAG	TACTCGAAATTTCCGGGGAC	50.0	50.0	58.3	57.3	411-432 bp	1.0	74.1