Hotspot

ndhC-trnM-CAU

LSC · 54996-56869 · 1874 bp

Back to Nekemias

Pi 0.00374

MarkerSeek score 61.50

Primer available yes

Label ndhC-trnM-CAU

Feature Metadata

label_name: ndhC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1874
variable_sites: 13
pi: 0.00374
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAU_p1	TGGTCCTTTACTAATCGGAGC	CCCGCCGTATGAAAGCAATA	47.6	50.0	57.2	58.4	2016-2033 bp	1.0	63.3
ndhC-trnM-CAU_p2	TGGTCCTTTACTAATCGGAGC	CCGCCGTATGAAAGCAATAC	47.6	50.0	57.2	57.3	2015-2032 bp	1.0	61.3
ndhC-trnM-CAU_p3	GGTCCTTTACTAATCGGAGCT	CCCGCCGTATGAAAGCAATA	47.6	50.0	56.9	58.4	2015-2032 bp	1.0	61.1
ndhC-trnM-CAU_p4	TGGTCCTTTACTAATCGGAGC	TTTGAACCAATGACTCCCGC	47.6	50.0	57.2	58.8	2031-2048 bp	1.0	60.6
ndhC-trnM-CAU_p5	CTGGTCCTTTACTAATCGGAGC	CCCGCCGTATGAAAGCAATA	50.0	50.0	58.3	58.4	2017-2034 bp	1.0	59.6