Hotspot

trnS-UGA-psbZ

LSC · 39067-39356 · 290 bp

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Pi 0
MarkerSeek score 21.80
Primer available yes
Label trnS-UGA-psbZ

Feature Metadata

label_name
trnS-UGA-psbZ
feature_type
igs
region
LSC
length_bp
290
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
21.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbZ_p1 CAAGACCGGAGCTATCAACC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.1 57.0 359 bp 1.0 71.6
trnS-UGA-psbZ_p2 CTATCAACCACTCGGCCATC TGCAAAAACAGCCAATTGGA 55.0 40.0 58.1 57.0 348 bp 1.0 71.0
trnS-UGA-psbZ_p3 CAAGACCGGAGCTATCAACC TGCAAAAACAGCCAATTGGAA 55.0 38.1 58.1 57.7 359 bp 1.0 69.4
trnS-UGA-psbZ_p4 CAAGACCGGAGCTATCAACC AAACAGCCAATTGGAAAGCA 55.0 40.0 58.1 56.7 354 bp 1.0 69.1
trnS-UGA-psbZ_p5 CAAGACCGGAGCTATCAACC AACAGCCAATTGGAAAGCAA 55.0 40.0 58.1 56.7 353 bp 1.0 69.1