Hotspot

psbM-trnD-GUC

LSC · 32136-33312 · 1177 bp

Back to Nekemias

Pi 0.00496

MarkerSeek score 62.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1177
variable_sites: 11
pi: 0.00496
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TGGGCTATGGCTATACACCT	45.0	50.0	55.8	57.6	1326-1329 bp	1.0	59.6
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	GTGGGCTATGGCTATACACC	45.0	55.0	55.8	57.5	1327-1330 bp	1.0	59.1
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1287-1290 bp	1.0	59.1
psbM-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	TGGGCTATGGCTATACACCT	40.9	50.0	57.4	57.6	1333-1336 bp	1.0	56.4
psbM-trnD-GUC_p5	AGGAACTAGAATGAACAGTGCA	GTGGGCTATGGCTATACACC	40.9	55.0	57.4	57.5	1334-1337 bp	1.0	55.9