Hotspot

rpoB-trnC-GCA

LSC · 28632-29839 · 1208 bp

Back to Nekemias

Pi 0.00304

MarkerSeek score 62.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1208
variable_sites: 7
pi: 0.00304
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CATTCCCTCATTTCCACCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.2	58.1	1295-1319 bp	1.0	78.8
rpoB-trnC-GCA_p2	CATTCCCTCATTTCCACCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.2	58.4	1277-1301 bp	1.0	76.6
rpoB-trnC-GCA_p3	ACATTCCCTCATTTCCACCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1296-1320 bp	1.0	76.3
rpoB-trnC-GCA_p4	GTTCTTCTGTCAAGCCCTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.4	58.1	1371-1395 bp	1.0	76.0
rpoB-trnC-GCA_p5	AGTTCTTCTGTCAAGCCCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.4	58.1	1372-1396 bp	1.0	76.0