Hotspot

ccsA

SSC · 118570-119535 · 966 bp

Back to Neillia

Pi 0.00145

MarkerSeek score 42.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 3
pi: 0.00145
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TCCTATAATTGAGGGACCCCC	CGTATGCCGTCCTATGTTTCT	52.4	47.6	58.0	57.9	1040-1046 bp	1.0	63.9
ccsA_p2	TCCTATAATTGAGGGACCCCC	CGTATGCCGTCCTATGTTTC	52.4	50.0	58.0	56.4	1040-1046 bp	1.0	60.0
ccsA_p3	CCTATAATTGAGGGACCCCC	CGTATGCCGTCCTATGTTTCT	55.0	47.6	56.4	57.9	1039-1045 bp	1.0	59.3
ccsA_p4	CCTATAATTGAGGGACCCCC	CGTATGCCGTCCTATGTTTC	55.0	50.0	56.4	56.4	1039-1045 bp	1.0	55.5
ccsA_p5	TCCTATAATTGAGGGACCCCC	CGTATGCCGTCCTATGTTTCTA	52.4	45.5	58.0	57.8	1040-1046 bp	1.0	54.8