Hotspot

rpl32-trnL-UAG

SSC · 117465-118395 · 931 bp

Back to Neillia

Pi 0.00892
MarkerSeek score 55.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
931
variable_sites
17
pi
0.00892
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGATATCGGGCAGCATTGAA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 1033-1050 bp 1.0 82.5
rpl32-trnL-UAG_p2 GGATATCGGGCAGCATTGAA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 1034-1051 bp 1.0 82.5
rpl32-trnL-UAG_p3 TTAAGGGAGAGAGGGGTTGG AGCGTGTCTACCGATTTCAC 55.0 50.0 58.0 58.0 1423-1440 bp 1.0 82.2
rpl32-trnL-UAG_p4 TTAAGGGAGAGAGGGGTTGG CAGCGTGTCTACCGATTTCA 55.0 50.0 58.0 58.0 1424-1441 bp 1.0 82.2
rpl32-trnL-UAG_p5 GGAGAGAGGGGTTGGGTTAT AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 1418-1435 bp 1.0 81.6