Hotspot

ndhF-rpl32

SSC · 116211-117305 · 1095 bp

Back to Neillia

Pi 0.00807

MarkerSeek score 52.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1095
variable_sites: 19
pi: 0.00807
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGTTGCCGTTGGAAAAAGT	TGAAATAGAGGTGCGTTTTT	40.0	35.0	57.0	53.4	1189-1229 bp	1.0	44.5
ndhF-rpl32_p2	TTGTTGCCGTTGGAAAAAGT	TTGAAATAGAGGTGCGTTTT	40.0	35.0	57.0	53.4	1190-1230 bp	1.0	44.5
ndhF-rpl32_p3	TTGTTGCCGTTGGAAAAAGT	TTTGAAATAGAGGTGCGTTT	40.0	35.0	57.0	53.4	1191-1231 bp	1.0	44.5
ndhF-rpl32_p4	TTGTTGCCGTTGGAAAAAGT	TTTTGAAATAGAGGTGCGTT	40.0	35.0	57.0	53.4	1192-1232 bp	1.0	44.5
ndhF-rpl32_p5	TTGTTGCCGTTGGAAAAAGT	TTTTTGAAATAGAGGTGCGT	40.0	35.0	57.0	53.4	1193-1233 bp	1.0	44.5