Hotspot

petD-rpoA

LSC · 81061-81311 · 251 bp

Back to Neillia

Pi 0.00763

MarkerSeek score 61.80

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 251
variable_sites: 4
pi: 0.00763
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GATTGGTACTGCAGTAGCCC	TGGACCTTTTGAATAACAGTCA	55.0	36.4	58.0	55.6	411-428 bp	1.0	48.2
petD-rpoA_p2	AGCCCTTTGGTTGGGTATTG	TGGACCTTTTGAATAACAGTCA	50.0	36.4	58.1	55.6	396-413 bp	1.0	48.2
petD-rpoA_p3	GGTTGGGTATTGGAGCAACA	TGGACCTTTTGAATAACAGTCA	50.0	36.4	58.1	55.6	388-405 bp	1.0	48.2
petD-rpoA_p4	TGGTTGGGTATTGGAGCAAC	TGGACCTTTTGAATAACAGTCA	50.0	36.4	58.1	55.6	389-406 bp	1.0	48.2
petD-rpoA_p5	GATTGGTACTGCAGTAGCCC	TGGACCTTTTGAATAACAGTCAAG	55.0	37.5	58.0	57.4	411-428 bp	1.0	46.6