Hotspot

rpl20-rps12

LSC · 72178-72975 · 798 bp

Back to Neillia

Pi 0.00753

MarkerSeek score 63.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 11
pi: 0.00753
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	AAATGTCACCAAATCCCCCG	50.0	50.0	57.9	58.4	952-954 bp	1.0	76.7
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	AAATGTCACCAAATCCCCCG	50.0	50.0	57.9	58.4	951-953 bp	1.0	76.7
rpl20-rps12_p3	TGTTCTACGTCTTCGGGCTA	AAATGTCACCAAATCCCCCG	50.0	50.0	58.2	58.4	908-910 bp	1.0	76.3
rpl20-rps12_p4	TTTGTTCTACGTCTTCGGGC	AAATGTCACCAAATCCCCCG	50.0	50.0	58.2	58.4	910-912 bp	1.0	76.0
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	CAGCGACGAGGAACATGTAC	50.0	55.0	57.9	58.7	914-916 bp	1.0	74.5