Hotspot

psbF-psbE

LSC · 67431-67439 · 9 bp

Back to Neillia

Pi 0.0444

MarkerSeek score 70.00

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: LSC
length_bp: 9
variable_sites: 1
pi: 0.0444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	GTCCGTGAACAGCTAACCAT	AACTGGCCGTTTTGATCCTT	50.0	45.0	57.9	58.0	122 bp	1.0	79.9
psbF-psbE_p2	GTCCGTGAACAGCTAACCAT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	57.9	58.0	121 bp	1.0	79.9
psbF-psbE_p3	TGGATGAACTGCATTGCTGA	AACTGGCCGTTTTGATCCTT	45.0	45.0	57.8	58.0	177 bp	1.0	78.8
psbF-psbE_p4	TGGATGAACTGCATTGCTGA	ACTGGCCGTTTTGATCCTTT	45.0	45.0	57.8	58.0	176 bp	1.0	78.8
psbF-psbE_p5	GAACAGCTAACCATCGCACT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.3	58.0	115 bp	1.0	78.5