Hotspot

atpH-atpI

LSC · 13740-14892 · 1153 bp

Back to Neillia

Pi 0.0047

MarkerSeek score 59.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1153
variable_sites: 11
pi: 0.0047
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.0	58.0	1206-1211 bp	1.0	80.6
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACCTTAGCTGCG	45.0	45.0	58.0	57.9	1236-1241 bp	1.0	79.7
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.2	58.0	1207-1212 bp	1.0	79.4
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTTGCAACCTTAGCTGCG	45.0	45.0	58.2	57.9	1237-1242 bp	1.0	78.6
atpH-atpI_p5	AGGTCCAATAGAAGCAAGCC	GCGAATCCATGGAAGGTCAT	50.0	50.0	57.6	58.0	1246-1251 bp	1.0	77.5