Hotspot

trnR-UCU-atpA

LSC · 9895-10207 · 313 bp

Back to Neillia

Pi 0.0179

MarkerSeek score 50.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 313
variable_sites: 10
pi: 0.0179
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCG	47.6	50.0	57.3	58.4	403-442 bp	1.0	69.3
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	GGAAGCCCTTTTGAAAGAAGC	47.6	47.6	57.3	58.0	384-423 bp	1.0	64.2
trnR-UCU-atpA_p3	GTTCAAATCCTATTGGACGCA	AGACATTTACCGACGAAGCG	42.9	50.0	56.6	58.4	402-441 bp	1.0	63.7
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ACCAAGACATTTACCGACGA	47.6	45.0	57.3	56.9	407-446 bp	1.0	63.4
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	TCAGGAACAGATGGAACGTT	47.6	45.0	57.3	56.8	360-399 bp	1.0	62.8