Hotspot

rps16-trnQ-UUG

LSC · 6344-6970 · 627 bp

Back to Neillia

Pi 0.0059
MarkerSeek score 58.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
627
variable_sites
8
pi
0.0059
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GCCGATCATGTCCTTCAAGT GCTATTCGGAGGTTCGAGTC 50.0 55.0 58.0 57.9 707-742 bp 1.0 82.5
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GCTATTCGGAGGTTCGAGTC 45.0 55.0 58.1 57.9 691-726 bp 1.0 81.8
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GCTATTCGGAGGTTCGAGTC 50.0 55.0 58.2 57.9 702-737 bp 1.0 81.2
rps16-trnQ-UUG_p4 GCCGATCATGTCCTTCAAGT GTGTCGACTAGAAATGGGGC 50.0 55.0 58.0 58.3 761-796 bp 1.0 80.5
rps16-trnQ-UUG_p5 GCCGATCATGTCCTTCAAGT TATTCGGAGGTTCGAGTCCT 50.0 50.0 58.0 57.6 705-740 bp 1.0 80.1