Hotspot

rbcL

LSC · 55266-56696 · 1431 bp

Back to Nassella

Pi 0.0014

MarkerSeek score 47.10

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1431
variable_sites: 3
pi: 0.0014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCAAAAGTTGTAGGGAGGGAC	AGAAGAATTACTGCATTTCGT	47.6	33.3	57.2	53.5	1543-1544 bp	1.0	40.2
rbcL_p2	TCAAAAGTTGTAGGGAGGGAC	AAGAAGAATTACTGCATTTCGT	47.6	31.8	57.2	54.4	1544-1545 bp	1.0	40.2
rbcL_p3	TTCAAAAGTTGTAGGGAGGGAC	AGAAGAATTACTGCATTTCGT	45.5	33.3	57.9	53.5	1544-1545 bp	1.0	40.2
rbcL_p4	CAAAAGTTGTAGGGAGGGACTT	AGAAGAATTACTGCATTTCGT	45.5	33.3	57.9	53.5	1542-1543 bp	1.0	40.2
rbcL_p5	TCAAAAGTTGTAGGGAGGGAC	AAAGAAGAATTACTGCATTTCGT	47.6	30.4	57.2	55.2	1545-1546 bp	1.0	40.2