Hotspot

atpF

LSC · 33488-34867 · 1380 bp

Back to Nassella

Pi 0.000483

MarkerSeek score 64.30

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1380
variable_sites: 1
pi: 0.000483
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GCGAGCGAAGTAAGTGATCT	TCGAAGGGTTGCCATTAGTG	50.0	50.0	57.8	57.9	1420-1551 bp	1.0	81.6
atpF_p2	GCGAGCGAAGTAAGTGATCT	CGAAGGGTTGCCATTAGTGT	50.0	50.0	57.8	58.2	1419-1550 bp	1.0	81.1
atpF_p3	GCGAGCGAAGTAAGTGATCT	GACTCGAAGGGTTGCCATTA	50.0	50.0	57.8	57.6	1423-1554 bp	1.0	79.3
atpF_p4	GCGAGCGAAGTAAGTGATCT	ACTCGAAGGGTTGCCATTAG	50.0	50.0	57.8	57.6	1422-1553 bp	1.0	79.3
atpF_p5	GCGAGCGAAGTAAGTGATCT	CTCGAAGGGTTGCCATTAGT	50.0	50.0	57.8	57.6	1421-1552 bp	1.0	79.3