Hotspot

ndhF-rpl32

SSC · 105588-106490 · 903 bp

Back to Nassella

Pi 0.00148

MarkerSeek score 59.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 903
variable_sites: 2
pi: 0.00148
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TAAGGCCAAACCCCATTGAC	TCCAAAGATTTTTACGAATACGCT	50.0	33.3	58.1	57.3	1043 bp	1.0	41.8
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TCCAAAGATTTTTACGAATACGCT	55.0	33.3	58.1	57.3	1014 bp	1.0	41.4
ndhF-rpl32_p3	GGCCAAACCCCATTGACATA	TCCAAAGATTTTTACGAATACGCT	50.0	33.3	58.1	57.3	1040 bp	1.0	41.2
ndhF-rpl32_p4	TAAGGCCAAACCCCATTGAC	TCCAAAGATTTTTACGAATACGC	50.0	34.8	58.1	55.9	1043 bp	1.0	40.2
ndhF-rpl32_p5	TAAGGCCAAACCCCATTGAC	CCAAAGATTTTTACGAATACGCT	50.0	34.8	58.1	55.9	1042 bp	1.0	40.2