Hotspot

rpl14-rpl16

LSC · 78682-78793 · 112 bp

Back to Nassella

Pi 0.0119

MarkerSeek score 60.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 112
variable_sites: 2
pi: 0.0119
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TCAATTTTCGCGCTCCACTA	TAGCTGCCAGTAAAATGCCC	45.0	50.0	57.9	58.2	223 bp	1.0	78.1
rpl14-rpl16_p2	AGCAGCTCCTATGACTCGAA	TAGCTGCCAGTAAAATGCCC	50.0	50.0	58.2	58.2	249 bp	1.0	76.9
rpl14-rpl16_p3	ACACATCAATTTTCGCGCTC	TAGCTGCCAGTAAAATGCCC	45.0	50.0	57.8	58.2	228 bp	1.0	76.8
rpl14-rpl16_p4	AGGGTCTGGGGTTGAATCAT	TAGCTGCCAGTAAAATGCCC	50.0	50.0	58.3	58.2	185 bp	1.0	76.5
rpl14-rpl16_p5	GCGCTCCACTATTATCTGCT	TAGCTGCCAGTAAAATGCCC	50.0	50.0	57.6	58.2	214 bp	1.0	75.3