Hotspot

ycf4-cemA

LSC · 59265-59698 · 434 bp

Back to Nassella

Pi 0.00309

MarkerSeek score 57.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 434
variable_sites: 2
pi: 0.00309
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ACAAAAAGCTGCCGAATTGG	CCCCAAGGCAAAAGTACCAT	45.0	50.0	57.8	58.1	552-554 bp	1.0	78.8
ycf4-cemA_p2	CCTATTTCTTGCGCGTACCA	CCCCAAGGCAAAAGTACCAT	50.0	50.0	58.4	58.1	532-534 bp	1.0	77.3
ycf4-cemA_p3	GCGCGTACCAATGGAAGTAT	CCCCAAGGCAAAAGTACCAT	50.0	50.0	58.4	58.1	522-524 bp	1.0	76.7
ycf4-cemA_p4	ATTTCTTGCGCGTACCAATG	CCCCAAGGCAAAAGTACCAT	45.0	50.0	57.5	58.1	529-531 bp	1.0	75.8
ycf4-cemA_p5	AATTGGCCTATTTCTTGCGC	CCCCAAGGCAAAAGTACCAT	45.0	50.0	57.4	58.1	538-540 bp	1.0	75.4