Hotspot

petN-trnC-GCA

LSC · 18085-18981 · 897 bp

Back to Nassella

Pi 0.000744

MarkerSeek score 59.50

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 897
variable_sites: 1
pi: 0.000744
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	944-946 bp	1.0	70.6
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	50.0	55.0	58.2	59.1	946-948 bp	1.0	70.2
petN-trnC-GCA_p3	CCACTCCTCCCCCATACTAC	AGTTCAAATCTGGGTGCCG	60.0	52.6	58.3	58.1	1000-1002 bp	1.0	69.6
petN-trnC-GCA_p4	CCACTCCTCCCCCATACTAC	CCAGTTCAAATCTGGGTGCC	60.0	55.0	58.3	59.1	1002-1004 bp	1.0	69.1
petN-trnC-GCA_p5	TCCACTCCTCCCCCATACTA	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.4	58.1	1001-1003 bp	1.0	68.8