Hotspot

psbZ-trnG-GCC

LSC · 11904-12120 · 217 bp

Back to Nassella

Pi 0.00307

MarkerSeek score 40.20

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 217
variable_sites: 1
pi: 0.00307
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TATTCCATCTAGCCGAGCCA	GTTTCCAGTACTCCAGAGCG	50.0	55.0	58.0	58.0	1598 bp	1.0	80.2
psbZ-trnG-GCC_p2	TATTCCATCTAGCCGAGCCA	GCAGTTTGCCAGTTGTTTGT	50.0	45.0	58.0	58.0	1257 bp	1.0	80.1
psbZ-trnG-GCC_p3	CAACCACTCAGCCATCTCTC	GTTTCCAGTACTCCAGAGCG	55.0	55.0	58.0	58.0	1430 bp	1.0	80.1
psbZ-trnG-GCC_p4	TATTCCATCTAGCCGAGCCA	GGGTGCAAAGGAAGGGTAAA	50.0	50.0	58.0	58.0	1162 bp	0.666667	66.7
psbZ-trnG-GCC_p5	CAACCACTCAGCCATCTCTC	GGGTGCAAAGGAAGGGTAAA	55.0	50.0	58.0	58.0	994 bp	0.666667	66.6