Hotspot

petA-psbJ

LSC · 65348-66383 · 1036 bp

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Pi 0.0779
MarkerSeek score 71.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1036
variable_sites
57
pi
0.0779
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.71
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGAGAAGGTTCAATTGTCCG ATTCCGGATTGGGTTCATCC 50.0 50.0 56.8 57.6 815-1096 bp 1.0 76.6
petA-psbJ_p2 CGAGAAGGTTCAATTGTCCGA ATTCCGGATTGGGTTCATCC 47.6 50.0 58.3 57.6 815-1096 bp 1.0 75.4
petA-psbJ_p3 TCGAGAAGGTTCAATTGTCCG ATTCCGGATTGGGTTCATCC 47.6 50.0 58.3 57.6 816-1097 bp 1.0 75.4
petA-psbJ_p4 CGAGAAGGTTCAATTGTCCG TGGTTCATATTCCGGATTGGG 50.0 47.6 56.8 57.8 823-1104 bp 1.0 69.7
petA-psbJ_p5 CGAGAAGGTTCAATTGTCCG GGTTCATATTCCGGATTGGGT 50.0 47.6 56.8 57.8 822-1103 bp 1.0 69.7