Hotspot

ycf4-cemA

LSC · 62964-63444 · 481 bp

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Pi 0.085
MarkerSeek score 83.20
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
481
variable_sites
39
pi
0.085
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TTGGCCTATTTCTTGCGTGT AGAAGAGGAGTGAATGCTTTCT 45.0 40.9 58.1 57.1 552-694 bp 1.0 63.8
ycf4-cemA_p2 AAAAAGCTGCTGAATTGGCC AGAAGAGGAGTGAATGCTTTCT 45.0 40.9 57.8 57.1 566-708 bp 1.0 63.0
ycf4-cemA_p3 AAAGCTGCTGAATTGGCCTA AGAAGAGGAGTGAATGCTTTCT 45.0 40.9 57.8 57.1 564-706 bp 1.0 62.7
ycf4-cemA_p4 TTGGCCTATTTCTTGCGTGT AGAAGAGGAGTGAATGCTTTCTT 45.0 39.1 58.1 57.8 552-694 bp 1.0 61.1
ycf4-cemA_p5 AGCTGCTGAATTGGCCTATT AGAAGAGGAGTGAATGCTTTCT 45.0 40.9 57.6 57.1 562-704 bp 1.0 60.9