Hotspot

accD-psaI

LSC · 61281-61878 · 598 bp

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Pi 0.0781
MarkerSeek score 77.00
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
598
variable_sites
40
pi
0.0781
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 ACCCGTTTGATCGAGTATGC CCATTGCAATTGCCGGAAAT 50.0 45.0 58.1 58.0 1090-1112 bp 1.0 86.7
accD-psaI_p2 ACCCGTTTGATCGAGTATGC TACATTTCAGACATCCGCCG 50.0 50.0 58.1 58.1 1368-1373 bp 1.0 86.7
accD-psaI_p3 ACCCGTTTGATCGAGTATGC ATTTCAGACATCCGCCGATC 50.0 50.0 58.1 58.1 1365-1370 bp 1.0 86.2
accD-psaI_p4 ACCCGTTTGATCGAGTATGC AAGAAGCCATTGCAATTGCC 50.0 45.0 58.1 57.9 1118 bp 0.5 58.7
accD-psaI_p5 GGTTCACAAGTGGCCGAATA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.2 58.0 806 bp 0.5 58.3