Hotspot

ndhC-trnV-UAC

LSC · 52807-53937 · 1131 bp

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Pi 0.0591
MarkerSeek score 79.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1131
variable_sites
63
pi
0.0591
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAGAAACA TTCGAGTCCGTATAGCCCTA 31.8 50.0 53.9 57.1 1142-1174 bp 1.0 48.4
ndhC-trnV-UAC_p2 CATATTCGTAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 33.3 55.0 52.3 58.7 1142-1174 bp 1.0 48.4
ndhC-trnV-UAC_p3 TCATATTCGTAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 31.8 55.0 53.9 58.7 1143-1175 bp 1.0 48.3
ndhC-trnV-UAC_p4 ATCATATTCGTAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 30.4 55.0 54.5 58.7 1144-1176 bp 1.0 48.3
ndhC-trnV-UAC_p5 TCATATTCGTAAAGCAGAAACAT GTTCGAGTCCGTATAGCCCT 30.4 55.0 54.5 58.7 1143-1175 bp 1.0 48.3