Hotspot

trnL-UAA-trnF-GAA

LSC · 50019-50381 · 363 bp

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Pi 0.0697
MarkerSeek score 78.60
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
363
variable_sites
23
pi
0.0697
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 AATCGTGAGGGTTCAAGTCC TCTACCAGCTGAGCTATCCC 50.0 55.0 57.5 58.0 387-417 bp 1.0 84.8
trnL-UAA-trnF-GAA_p2 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 423-453 bp 1.0 84.0
trnL-UAA-trnF-GAA_p3 AATCGTGAGGGTTCAAGTCC TCAGTCCTCTGCTCTACCAG 50.0 55.0 57.5 57.9 399-429 bp 1.0 83.6
trnL-UAA-trnF-GAA_p4 AATCGTGAGGGTTCAAGTCC GTGCCAGGAACCAGATTTGA 50.0 50.0 57.5 58.1 438-468 bp 1.0 83.5
trnL-UAA-trnF-GAA_p5 GAGTCCCATTCCACATGTCA TCTACCAGCTGAGCTATCCC 50.0 55.0 57.2 58.0 458-488 bp 1.0 81.6