Hotspot

trnR-UCU-atpA

LSC · 10796-10926 · 131 bp

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Pi 0.12
MarkerSeek score 81.40
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
131
variable_sites
15
pi
0.12
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 ATCGGAATCAAAAGCGTCCA CAGGAACAGATGGAGCGTTT 45.0 50.0 57.9 58.2 254-258 bp 1.0 84.2
trnR-UCU-atpA_p2 TCGGAATCAAAAGCGTCCAT CAGGAACAGATGGAGCGTTT 45.0 50.0 57.9 58.2 253-257 bp 1.0 84.2
trnR-UCU-atpA_p3 TCGGAATCAAAAGCGTCCAT ATTCAGGAACAGATGGAGCG 45.0 50.0 57.9 57.7 256-260 bp 1.0 83.1
trnR-UCU-atpA_p4 ATCGGAATCAAAAGCGTCCA ATTCAGGAACAGATGGAGCG 45.0 50.0 57.9 57.7 257-261 bp 1.0 83.0
trnR-UCU-atpA_p5 TCGGAATCAAAAGCGTCCAT AGGAACAGATGGAGCGTTTT 45.0 45.0 57.9 57.4 252-256 bp 1.0 81.2