Hotspot

trnK-UUU-rps16

LSC · 4235-5090 · 856 bp

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Pi 0.0509
MarkerSeek score 77.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
856
variable_sites
41
pi
0.0509
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1257-1280 bp 1.0 85.3
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1256-1279 bp 1.0 85.3
trnK-UUU-rps16_p3 CCCTTTCAGGATCAGTCGTG TCATTTTTGCTCGAGCCGTA 55.0 45.0 58.0 57.9 1271-1294 bp 1.0 85.2
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG TCATTTTTGCTCGAGCCGTA 55.0 45.0 58.0 57.9 1270-1293 bp 1.0 85.2
trnK-UUU-rps16_p5 TCGACCCAAACTCGTTTTGT GCCGTACGAGGAGAAAACTT 45.0 50.0 58.0 57.9 1381-1400 bp 1.0 84.8