Hotspot

trnH-GUG-psbA

LSC · 77-403 · 327 bp

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Pi 0.0801
MarkerSeek score 77.20
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
327
variable_sites
25
pi
0.0801
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TGAAGCTCCATCTACAAATGG 55.0 42.9 58.1 55.7 374-378 bp 1.0 60.7
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TGAAGCTCCATCTACAAATGG 50.0 42.9 57.9 55.7 388-392 bp 1.0 60.4
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TGAAGCTCCATCTACAAATGG 50.0 42.9 57.9 55.7 387-391 bp 1.0 60.4
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TGAAGCTCCATCTACAAATGG 50.0 42.9 57.9 55.7 389-393 bp 1.0 60.4
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG TTGAAGCTCCATCTACAAATGG 55.0 40.9 58.1 56.4 375-379 bp 1.0 58.7