Hotspot

ndhF

SSC · 114601-116814 · 2214 bp

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Pi 0.0154

MarkerSeek score 54.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2214
variable_sites: 83
pi: 0.0154
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TATTGCAATTCCCCGAGTGG	TTTGTTCCCTTCGAAAGCGA	50.0	45.0	57.9	58.0	2591-2612 bp	0.571429	64.1
ndhF_p2	GGAAGGGAAGCAGAACTAGC	TTTGTTCCCTTCGAAAGCGA	55.0	45.0	58.0	58.0	2349-2358 bp	0.428571	57.6
ndhF_p3	GGAAGGGAAGCAGAACTAGC	CAGTTCCGCAATGAGTCAGA	55.0	50.0	58.0	57.9	2751-2764 bp	0.428571	57.6
ndhF_p4	GGAAGGGAAGCAGAACTAGC	TCAGTTCCGCAATGAGTCAG	55.0	50.0	58.0	57.9	2752-2765 bp	0.428571	57.5
ndhF_p5	AACTAGCTTTTGGGTCTGCC	TTTGTTCCCTTCGAAAGCGA	50.0	45.0	58.1	58.0	2341 bp	0.142857	44.7