Hotspot

atpF

LSC · 12522-14026 · 1505 bp

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Pi 0.02

MarkerSeek score 64.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1505
variable_sites: 68
pi: 0.02
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CCGAGATTCAGAAGCGGAAA	GGGCTGATTTGAGGATGAGG	50.0	55.0	58.0	58.0	2223-2319 bp	1.0	87.1
atpF_p2	CCGAGATTCAGAAGCGGAAA	CTCGTTGCCATTTGCTTCTG	50.0	50.0	58.0	58.0	2303-2400 bp	1.0	87.1
atpF_p3	CCGAGATTCAGAAGCGGAAA	ATTTATGGACTGGTCGTGGC	50.0	50.0	58.0	58.0	2427-2524 bp	1.0	87.1
atpF_p4	AAATAAGCCTCGCTCACTGG	GGGCTGATTTGAGGATGAGG	50.0	55.0	58.0	58.0	2153-2249 bp	0.857143	81.2
atpF_p5	CCAAATAAGCCTCGCTCACT	GGGCTGATTTGAGGATGAGG	50.0	55.0	58.0	58.0	2155-2251 bp	0.857143	81.2