Hotspot

ccsA-ndhD

SSC · 119821-120013 · 193 bp

Back to Narcissus

Pi 0.0449

MarkerSeek score 65.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 193
variable_sites: 20
pi: 0.0449
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ACCTGCCCTACAATCTCAGT	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.0	58.0	1133 bp	1.0	83.4
ccsA-ndhD_p2	ACCTGCCCTACAATCTCAGT	CTCGATGGCTTCTCTTGCAT	50.0	50.0	58.0	58.0	1131 bp	1.0	83.1
ccsA-ndhD_p3	ACCTGCCCTACAATCTCAGT	TTCTTGGTGGGAACGAGTTG	50.0	50.0	58.0	58.0	1235 bp	1.0	82.9
ccsA-ndhD_p4	TTTTACCCAAGGTTTCGCCA	TTCTCGATGGCTTCTCTTGC	45.0	50.0	57.9	58.0	1196 bp	0.857143	77.1
ccsA-ndhD_p5	ACCTGCCCTACAATCTCAGT	TGTCCCTGGTTGGTGATAGT	50.0	50.0	58.0	58.0	1445 bp	0.857143	76.5