Hotspot

rpl32-trnL-UAG

SSC · 117912-118696 · 785 bp

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Pi 0.0246

MarkerSeek score 69.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 785
variable_sites: 46
pi: 0.0246
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACAAACAAGTAAGAAAATCTTGGA	TCCTAAGAGCAGCGTGTCTA	29.2	50.0	55.1	57.9	768-860 bp	1.0	48.4
rpl32-trnL-UAG_p2	ACAAACAAGTAAGAAAATCTTGGA	AGCAGCGTGTCTACCAATTT	29.2	45.0	55.1	57.8	761-853 bp	1.0	48.4
rpl32-trnL-UAG_p3	ACAAACAAGTAAGAAAATCTTGGA	CCTAAGAGCAGCGTGTCTAC	29.2	55.0	55.1	57.8	767-859 bp	1.0	48.4
rpl32-trnL-UAG_p4	ACAAACAAGTAAGAAAATCTTGGA	CTAAGAGCAGCGTGTCTACC	29.2	55.0	55.1	57.8	766-858 bp	1.0	48.4
rpl32-trnL-UAG_p5	ACAAACAAGTAAGAAAATCTTGGA	GAGCAGCGTGTCTACCAATT	29.2	50.0	55.1	58.3	762-854 bp	1.0	48.4