Hotspot

ndhF-rpl32

SSC · 116815-117737 · 923 bp

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Pi 0.0242
MarkerSeek score 65.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
923
variable_sites
55
pi
0.0242
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACTGGAAACGGAAGAAAAGGT AGAAAAAGCTTTTATGGCAGC 42.9 38.1 57.7 55.6 906-1075 bp 1.0 51.1
ndhF-rpl32_p2 ACTGGAAACGGAAGAAAAGGT AAGAAAAAGCTTTTATGGCAGC 42.9 36.4 57.7 56.4 907-1076 bp 1.0 49.1
ndhF-rpl32_p3 ACTGGAAACGGAAGAAAAGGT GAAAAAGCTTTTATGGCAGC 42.9 40.0 57.7 54.1 905-1074 bp 1.0 48.3
ndhF-rpl32_p4 ACTGGAAACGGAAGAAAAGGT AAAGAAAAAGCTTTTATGGCAGC 42.9 34.8 57.7 57.1 908-1077 bp 1.0 48.3
ndhF-rpl32_p5 ACTGGAAACGGAAGAAAAGGT AGAAAAAGCTTTTATGGCAGCTA 42.9 34.8 57.7 57.0 906-1075 bp 1.0 48.3