Hotspot

rpl22-rps19

IRb · 86023-86076 · 54 bp

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Pi 0.0476

MarkerSeek score 70.80

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 54
variable_sites: 7
pi: 0.0476
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	ACAAAAGGACCTCCGTTGAC	TCTGACTTTCGCGAGACATG	50.0	50.0	58.0	58.0	367-372 bp	0.857143	79.1
rpl22-rps19_p2	CACAAAAGGACCTCCGTTGA	TCTGACTTTCGCGAGACATG	50.0	50.0	58.0	58.0	368-373 bp	0.857143	79.0
rpl22-rps19_p3	TCACAAAAGGACCTCCGTTG	TCTGACTTTCGCGAGACATG	50.0	50.0	58.0	58.0	369-374 bp	0.857143	79.0
rpl22-rps19_p4	ACAAAAGGACCTCCGTTGAC	GTTATCCTGCGCTTGGAAGA	50.0	50.0	58.0	58.0	932-937 bp	0.857143	76.2
rpl22-rps19_p5	CACAAAAGGACCTCCGTTGA	GTTATCCTGCGCTTGGAAGA	50.0	50.0	58.0	58.0	933-938 bp	0.857143	76.2