Hotspot

atpB-rbcL

LSC · 56954-57871 · 918 bp

Back to Narcissus

Pi 0.0261

MarkerSeek score 71.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 918
variable_sites: 60
pi: 0.0261
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ACCGGAAGTAGTAGGATTGGT	AGTCTCTGTTTGTGGTGACA	47.6	45.0	57.9	56.6	956-980 bp	1.0	68.5
atpB-rbcL_p2	ACCGGAAGTAGTAGGATTGGT	AGTCTCTGTTTGTGGTGACAT	47.6	42.9	57.9	57.2	956-980 bp	1.0	64.7
atpB-rbcL_p3	ACCGGAAGTAGTAGGATTGGT	TGCTTTAGTCTCTGTTTGTGGT	47.6	40.9	57.9	57.8	962-986 bp	1.0	62.3
atpB-rbcL_p4	ACCGGAAGTAGTAGGATTGG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	56.0	56.6	956-980 bp	1.0	61.8
atpB-rbcL_p5	CCGGAAGTAGTAGGATTGGT	AGTCTCTGTTTGTGGTGACA	50.0	45.0	56.0	56.6	955-979 bp	1.0	61.8